%A Gao,Li %A Chen,Gang %A Liang,Zi-Qian %A Li,Jian-Di %A Li,Dong-Ming %A Tang,Yu-Lu %A Tang,Deng %A Huang,Zhi-Guang %A Chen,Jun-Hong %A Luo,Jia-Yuan %A Zeng,Jiang-Hui %A Dang,Yi-Wu %A Feng,Zhen-Bo %D 2022 %J Pathology and Oncology Research %C %F %G English %K molecular mechanism,endometrial carcinoma,RNA-seq,CKS2,in-house tissue microarray %Q %R 10.3389/pore.2022.1610307 %W %L %M %P %7 %8 2022-May-27 %9 Original Research %# %! CKS2 in endometrial carcinoma %* %< %T Expression Profile and Molecular Basis of Cyclin-Dependent Kinases Regulatory Subunit 2 in Endometrial Carcinoma Detected by Diversified Methods %U https://www.por-journal.com/articles/10.3389/pore.2022.1610307 %V 28 %0 JOURNAL ARTICLE %@ 1532-2807 %X Purpose: Our purpose was to systematically appraise the clinicopathological significance and explore the molecular bases of CKS2 in endometrial carcinoma.Patients and Methods: We measured the clinicopathological significance of CKS2 using diverse methods of public RNA-seq, microarrays, and in-house tissue microarrays to investigate the molecular basis of CKS2 in endometrial carcinoma through upstream transcriptional analysis, immune infiltration correlation analysis, and co-expression analysis.Results: Both the analysis for public RNA-seq plus the microarray data and in-house tissue microarray confirmed the significant overexpression of CKS2 in a total of 1,021 endometrial carcinoma samples compared with 279 non-cancer endometrium samples (SMD = 2.10, 95% CI = 0.72–3.48). The upregulated CKS2 was significantly related to the lymph node metastasis and advanced clinical grade of endometrial carcinoma patients (p < 0.001). Mutation types such as amplification and mRNA occurred with high frequency in the CKS2 gene in endometrial carcinoma patients. A series of miRNAs and transcription factors, such as hsa-miR-26a, hsa-miR-130a, hsa-miR-30, E2F4, MAX, and GABPA, were predicted to regulate the transcription and expression of CKS2. Significant links were found between CKS2 expression and the infiltration level of B cells, CD4+ T cells, and neutrophils in endometrial carcinoma. CKS2-coexpressed genes were actively involved in pathways such as the mitotic cell cycle process, PID aurora B pathway, and prolactin signaling pathway.Conclusion: The overexpressed CKS2 showed positive correlations with the clinical progression of endometrial carcinoma and was associated with various cancer-related biological processes and pathways, showing potential as a promising clinical biomarker for endometrial carcinoma.