AUTHOR=Gao Li , Chen Gang , Liang Zi-Qian , Li Jian-Di , Li Dong-Ming , Tang Yu-Lu , Tang Deng , Huang Zhi-Guang , Chen Jun-Hong , Luo Jia-Yuan , Zeng Jiang-Hui , Dang Yi-Wu , Feng Zhen-Bo 

TITLE=Expression Profile and Molecular Basis of Cyclin-Dependent Kinases Regulatory Subunit 2 in Endometrial Carcinoma Detected by Diversified Methods

JOURNAL=Pathology and Oncology Research

VOLUME=Volume 28 - 2022

YEAR=2022

URL=https://www.por-journal.com/journals/pathology-and-oncology-research/articles/10.3389/pore.2022.1610307

DOI=10.3389/pore.2022.1610307

ISSN=1532-2807

ABSTRACT=Purpose: To systematically appraise the clinico-pathological significance and explore the molecular bases of CKS2 in endometrial carcinoma. 
Patients and methods: We measured the clinico-pathological significance of CKS2 with diverse methods of public RNA-seq, microarrays and in-house tissue microarray and to investigate the molecular basis of CKS2 in endometrial carcinoma through upstream transcriptional analysis, immune infiltration correlation analysis and co-expression analysis. 
Results: Both analysis for public RNA-seq plus microarray data and in-house tissue microarray confirmed the significant overexpression of CKS2 in a total of 1021 endometrial carcinoma samples compared with 279 non-cancer endometrium samples (SMD=2.10, 95% CI= 0.72-3.48). The up-regulated CKS2 was significantly related to the lymph node metastasis and advanced clinical grade of endometrial carcinoma patients (P<0.001). Mutation types such as amplification and mRNA high frequently occurred in CKS2 gene in endometrial carcinoma patients. A series of miRNAs and transcription factors such as hsa-miR-26a, hsa-miR-130a, hsa-miR-30, E2F4, MAX and GABPA were predicted to regulate the transcription and expression of CKS2. Significant links were found between CKS2 expression and the infiltration level of B cell, CD4+ T cell and neutrophils in endometrial carcinoma. CKS2-coexpressed genes were actively involved in pathways such as mitotic cell cycle process, PID aurora B pathway and prolactin signaling pathway. 
Conclusion: The overexpressed CKS2 showed positive correlations with the clinical progression of endometrial carcinoma and was associated with various cancer-related biological processes and pathways, presenting the potential as promising clinical biomarker for endometrial carcinoma.