%A Wang,Linlin %A Liu,Dan %A Wei,Jun %A Yuan,Liwei %A Zhao,Shiyun %A Huang,Yani %A Ma,Jingwen %A Yang,Zhijuan %D 2021 %J Pathology and Oncology Research %C %F %G English %K molecular biology,miRNA-543,MAPK1,Wnt/β-catenin,endometrial stromal cell %Q %R 10.3389/pore.2021.1609761 %W %L %M %P %7 %8 2021-April-29 %9 Original Research %+ Mx Jun Wei,Ningxia Medical University,Yinchuan,750004,Ningxia,China,weijun8876@hotmail.com %# %! Role of miR-543 in intrauterine adhesion %* %< %T MiR-543 Inhibits the Migration and Epithelial-To-Mesenchymal Transition of TGF-β-Treated Endometrial Stromal Cells via the MAPK and Wnt/β-Catenin Signaling Pathways %U https://www.por-journal.com/articles/10.3389/pore.2021.1609761 %V 27 %0 JOURNAL ARTICLE %@ 1532-2807 %X Intrauterine adhesion (IUA) is one of the most prevalent reproductive system diseases in females. MicroRNAs (miRNAs) are reported to be master regulators in a variety of diseases, including IUA, but the role of microRNA-543 (miR-543) in IUA remains to be elucidated. In this study, we observed that miR-543 was downregulated in transforming growth factor-beta (TGF-β)-treated endometrial stromal cells (ESCs). Functionally, we observed that miR-543 suppressed the migration, epithelial-to-mesenchymal transition (EMT), and inhibited expression of extracellular matrix (ECM) proteins in TGF-β-treated ESCs. Mechanistically, MAPK1 is targeted by miR-543 after prediction and screening. A luciferase reporter assay demonstrated that miR-543 complementarily binds with the 3′ untranslated region of mitogen-activated protein kinase 1 (MAPK1), and western blot analysis indicated that miR-543 negatively regulates MAPK1 protein levels. In addition, results from rescue assays showed that miR-543 inhibits the migration and EMT of TGF-β-treated ESCs by targeting MAPK1. In addition, we observed that miR-543 inactivates the Wnt/β-catenin signaling pathway through inhibiting the phosphorylation of MAPK1 and β-catenin. Finally, we confirmed that miR-543 represses migration, EMT and inhibits levels of ECM proteins in TGF-β-treated ESCs by targeting the Wnt/β-catenin signaling pathway. Our results demonstrated that miR-543 suppresses migration and EMT of TGF-β-treated ESCs by targeting the MAPK and Wnt/β-catenin pathways.