A case report of recurrent leiomyosarcoma with chondrosarcoma differentiation in the abdominal wall and a review of the literature

Abstract

Leiomyosarcoma with heterologous differentiation is relatively rare. To date, only 19 cases have been reported in the English literature. Heterologous components frequently show histological pleomorphism, while those exhibiting well-differentiated morphology are seldom reported. Here, we report a 34-year-old female diagnosed with leiomyosarcoma and developed abdominal wall recurrence 8 years after primary surgery. The recurrent tumor mainly comprised well-differentiated chondrosarcoma except a single focus of leiomyosarcoma. Due to the rarity and prolonged onset of such a transition, our case provides insight into the understanding of this phenomenon.

Case report

A 34-year-old woman presented to our hospital with concerns of an abdominal mass with dull pain for several days. Physical examination found a palpable mass measuring 5–6 cm in diameter in the lower abdomen. Computed tomography revealed a multinodular mass measuring 7.6 cm × 4.1 cm × 6.5 cm located in the rectus abdominis muscle with multifocal calcification and uneven enhancement. Serum tumor markers such as CA125 (Cancer Antigen 125), SCC (Squamous Cell Carcinoma Antigen), and CA19-9 (Cancer Antigen 19-9) were all in the normal range. She had a history of “broad ligament leiomyosarcoma” 8 years prior and was treated with radical resection in our hospital. Because recurrence was suspected, she underwent a second surgery for abdominal mass excision. A tough and irregular mass was found beneath the lower abdominal wall during the operation. The mass invaded the subcutaneous fat layer and penetrated through the rectus abdominis muscle. No other intraoperative abnormities were found.

Grossly, the tumor had a multinodular appearance in the abdominal muscles below the superficial fascia and intruded into the extraperitoneal adipose tissue. Nodules measuring from 4 to 9 cm were found isolated from each other. All nodules were irregular in shape, with sharp margins and well-demarcated outlines. The cut surfaces were all cartilage-like with a brittle texture, gray to gray‒white color and focal calcification (Figure 1).

FIGURE 1

Microscopically, the vast majority of the neoplasm was composed of well-differentiated cartilaginous islands arranged in lobules (Figure 2A). The boundaries were generally smooth. The chondrocytes were uniform in shape with mild to moderate atypia and were unevenly distributed in the cartilage matrix (Figures 2B, C). Aside from the cartilage islands, a single cluster of spindle cells was observed (Figure 2D). The spindle cells were arranged in bundles and featured cigar-shaped nuclei and eosinophilic cytoplasm (Figure 2E). The spindle cells were positive for ER (Estrogen Receptor), PR (Progesterone Receptor), Caldesmon and Desmin, indicating an origin of smooth muscle cells from the genital tract. The strong positivity of P53 in chondrocytes indicated that cartilage islands, although well differentiated, were malignant (Figure 2).

FIGURE 2

To clarify the relationship between recurrent and primary tumors, we reviewed the histomorphology of the former surgical specimen. The primary tumor was composed of two types of cells: spindle cells and epithelioid cells. Sarcomatoid-like spindle cells were arranged in the fasciculus or vortex with cigar-shaped nuclei and sparse eosinophilic cytoplasm under high magnification (Figure 3A). Epithelioid cells with round to ovoid nuclei and prominent nucleoli arranged in cords or small nests were embedded in the edematous and mucinous matrix (Figure 3B). Marked necrosis could be easily observed under low-power microscopy (Figure 3C). The tumor cells showed overall mild to moderate atypia with active mitosis demonstrating over 10/10 HPF (Figures 3D, E). Such morphological characteristics met the standard for a diagnosis of leiomyosarcoma. It is worth mentioning that no heterologous component was discovered after thorough evaluation.

FIGURE 3

The final diagnosis was leiomyosarcoma with epithelioid differentiation. The patient then received 4 cycles of APDTIC (EPB, DTIC, DDP) chemotherapy after radical surgery.

The immunoprofiles of primary and recurrent tumor were listed in Table 1. As shown below, tumor cells in both lesions were positive for Vimentin, ER, PR, Caldesmon, SMA (Smooth Muscle Actin), Desmin, and negative for P-CK (pan Cytokeratin). P16 was found patchy in both lesions. The Ki67 index was approximately 25% in the primary neoplasm. Although it was only 2% in the recurrent neoplasm, the increased expression of P53 indicated malignant biological behavior of the recurrent tumor.

Taking into account both the overall medical history and the histopathological characteristics, the abdominal lesion was confirmed to be leiomyosarcoma with chondrosarcoma differentiation. The patient was in good condition after 6 cycles of combined chemotherapy (pharmorubicin, cis-platinum and dacarbazine).

Discussion

Although leiomyosarcoma is the most common malignant mesenchymal tumor of the uterus, such lesions containing heterologous components are rare, with fewer than 20 cases reported to date (1–9). The clinicopathological characteristics are summarized in Table 2. These tumors mainly affect postmenopausal women. The age of onset is reported to range from 41 to 80 years, with an average age of 53 years. Most patients present with an abdominal mass accompanied by dull pain or vaginal bleeding and irregular menstrual cycles. Most tumors are lobular or nodular, with a median size exceeding 15 cm in diameter (4). The appearance of the cut surface depends on the property and proportion of the heterologous components. Necrosis and hemorrhage are common. These tumors display highly malignant clinical courses and can show remote recurrences (10). Almost all patients experience metastasis or local recurrences (16/19). Lung is the most common recurrence site. The relapse interval varies from 5 months to 9 years. Pathologically, heterologous components can be seen not only in primary lesions but also in metastases. Osteosarcomatous differentiation is most frequent. Most of the heterologous components are poorly differentiated. Notably, such transitions occur mainly in typical leiomyosarcoma, except for one report of a myxoid leiomyosarcoma (1). Whether these changes could occur in pure epithelioid leiomyosarcoma has not yet been reported.

In our case, the patient was initially diagnosed with leiomyosarcoma with epithelioid differentiation and experienced distant recurrence. The recurrent tumor was composed mainly of well-differentiated chondrosarcoma. Misdiagnosis can easily occur regardless of the immunotype and the patient’s medical history. The differential diagnosis mainly depends on the proportion and histologic type of the heterologous component. Cases in which chondrosarcoma and osteosarcoma are the major components, careful sampling and detailed observations are mandatory to discover the smooth muscle component. For leiomyosarcoma with liposarcoma differentiation, the differential diagnosis from dedifferentiated liposarcoma might be challenging. In such cases, molecular tests can provide helpful evidence, as liposarcoma is characterized by the amplification of MDM2 (11, 12). When two or more unrelated differentiated histology types are confirmed in a single entity, malignant mesenchymoma should be considered (13). However, as most cases can be reclassified, the nomenclature is no longer applied. It is worth noting that sometimes heterologous components may be well differentiated and even mask the nature of the neoplasm. For this reason, a thorough review of the clinical information, careful sampling and skillful pathological examinations are imperative for diagnosis. The overall disease course tends to be aggressive, regardless of the histologic type and the degree of differentiation. More importantly, such tumors respond poorly to combination therapies and could develop late metastases. The mechanism underlying the development of heterologous components is likely to be the reacquisition of stem cell features and the triggering of mesenchymal primordium differentiation (14). An accumulation of similar cases may allow for additional in-depth studies to provide a basis for investigating pathogenetic mechanisms and biological behavior.

The remote metastasis that occurred in our case, although after a long remission interval, urges us to pay sufficient attention to patients who are clinically stable. Regardless the proportion of the heterologous components, the diagnosis of a de novo malignancy can only be established if recurrence is completely excluded.

References

• 1.

  ThiryayiSATurashviliGLattaEKSwansonDZhangLAntonescuCRet alPLAG1-rearrangment in a uterine leiomyosarcoma with myxoid stroma and heterologous differentiation. Genes Chromosomes Cancer (2021) 60(10):713–7. 10.1002/gcc.22980

  • CrossRef
  • Google Scholar

• 2.

  BapatKBrusteinS. Uterine sarcoma with liposarcomatous differentiation: Report of a case and review of the literature. Int J Gynaecol Obstet (1989) 28(1):71–5. 10.1016/0020-7292(89)90547-x

  • CrossRef
  • Google Scholar

• 3.

  DamjanovIFanF. Chondrosarcomatous differentiation of a metastatic leiomyosarcoma of the uterus. Virchows Arch (2006) 449(4):493–4. 10.1007/s00428-006-0283-5

  • CrossRef
  • Google Scholar

• 4.

  YuSHornickJL. "Malignant mesenchymoma" revisited: A clinicopathologic study of leiomyosarcomas with osteosarcomatous differentiation. Am J Surg Pathol (2022) 46(10):1430–5. 10.1097/PAS.0000000000001928

  • CrossRef
  • Google Scholar

• 5.

  ParikhPMaheshwariARekhiB. Two uncommon cases of uterine leiomyosarcomas displaying heterologous osteosarcomatous de-differentiation. J Cancer Res Ther (2015) 11(3):654. 10.4103/0973-1482.140984

  • CrossRef
  • Google Scholar

• 6.

  CappucciniFTewariKFullerPNPindurADiSaiaPJ. Aggressive clinical behavior of a rare uterine sarcoma. Gynecol Oncol (1998) 70(1):147–51. 10.1006/gyno.1998.5020

  • CrossRef
  • Google Scholar

• 7.

  BoyrazBGogakosTRaskinKANielsenGP. Metastatic uterine leiomyosarcoma with rhabdomyosarcomatous heterologous differentiation. Int J Gynecol Pathol (2022) 42:151–4. 10.1097/PGP.0000000000000874

  • CrossRef
  • Google Scholar

• 8.

  Anh TranTHollowayRW. Metastatic leiomyosarcoma of the uterus with heterologous differentiation to malignant mesenchymoma. Int J Gynecol Pathol (2012) 31(5):453–7. 10.1097/PGP.0b013e318246977d

  • CrossRef
  • Google Scholar

• 9.

  Den BakkerMAHegtVNSleddensHBNuijtenASMDinjensWNM. Malignant mesenchymoma of the uterus, arising in a leiomyoma. Histopathology (2002) 40(1):65–70. 10.1046/j.1365-2559.2002.01290.x

  • CrossRef
  • Google Scholar

• 10.

  SetiaAKanotraSAggarwalRBhavthankarDP. Epithelioid leiomyosarcoma of uterus [J]. BMJ Case Rep (2012) 2012:bcr1120115144. 10.1136/bcr.11.2011.5144

  • CrossRef
  • Google Scholar

• 11.

  ThwayKJonesRLNoujaimJZaidiSMiahABFisherC. Dedifferentiated liposarcoma: Updates on morphology, genetics, and therapeutic strategies. Adv Anat Pathol (2016) 23(1):30–40. 10.1097/PAP.0000000000000101

  • CrossRef
  • Google Scholar

• 12.

  LeeATJThwayKHuangPHJonesRL. Clinical and molecular spectrum of liposarcoma. J Clin Oncol (2018) 36(2):151–9. 10.1200/JCO.2017.74.9598

  • CrossRef
  • Google Scholar

• 13.

  StoutAP. Mesenchymoma, the mixed tumor of mesenchymal derivatives. Ann Surg (1948) 127(2):278–90. 10.1097/00000658-194802000-00007

  • CrossRef
  • Google Scholar

• 14.

  BuonomoFBussolaroSGiordaGRomanoFBiffiSRicciG. Cotyledonoid leiomyoma clinical characteristics, imaging features, and review of the literature. J Ultrasound Med (2021) 40(7):1459–69. 10.1002/jum.15510

  • CrossRef
  • Google Scholar