TY - JOUR AU - Huang, Qiang AU - Shen, Yu-Jie AU - Hsueh, Chi-Yao AU - Zhang, Yi-Fan AU - Yuan, Xiao-Hui AU - Zhou, Yu-Juan AU - Li, Jiao-Yu AU - Lin, Lan AU - Wu, Chun-Ping AU - Hu, Chun-Yan PY - 2022 M3 - Original Research TI - Plasma Extracellular Vesicles-Derived miR-99a-5p: A Potential Biomarker to Predict Early Head and Neck Squamous Cell Carcinoma JO - Pathology and Oncology Research UR - https://www.por-journal.com/articles/10.3389/pore.2022.1610699 VL - 28 SN - 1532-2807 N2 - Purpose: This study aimed to investigate the applicability of plasma extracellular vesicles (EVs) miR-99a-5p as a potential head and neck squamous cell carcinoma (HNSCC) diagnostic biomarker.Methods: The miRNA expression of HNSCC tissue and plasma EVs were profiled by small RNA sequencing. qRT-PCR was performed to detect miR-99a-5p expression in HNSCC (n = 93) and benign disease (n = 39) plasma EVs and formalin-fixed and paraffin-embedded (FFPE) tissue (n = 110). We constructed receiver-operating characteristic curves to investigate the diagnostic efficiency of plasma EVs miR-99a-5p.Results: Tumor tissue exhibited lower miR-99a-5p than para-tumor tissue. Patients with high miR-99a-5p expression exhibited significantly more p16 positive status. In contrast, HNSCC plasma EVs harbored more miR-99a-5p than the benign disease group. Plasma EVs miR-99a-5p distinguished HNSCC with area under the curve (AUC) of 0.7494 (95% CI: 0.6692–0.8296; p < 0.0001), with 61.54% sensitivity and 75.27% specificity, respectively. Furthermore, plasma EVs miR-99a-5p also distinguished early HNSCC with AUC of 0.7394 (95% CI: 0.6284–0.8504; p = 0.0002), with 79.07% sensitivity and 61.54% specificity, respectively.Conclusion: Plasma EVs miR-99a-5p is a potential biomarker for predicting early HNSCC. ER -