The Research Progress of Direct KRAS G12C Mutation Inhibitors

Abstract: KRAS mutations have long been considered undruggable. However, a series of direct KRAS mutation inhibitors have been developed since the switch II pocket was discovered recently. This review will summarize progress in the development of direct KRAS G12C mutation inhibitors, current relevant drugs under study and challenges that need to be considered in future research.

dramatically enhanced by GTPase-activating proteins (GAPs). For additional details, see Figure 3.
However, mutations at codons 12 and 13 in RAS proteins impair GAP-stimulated GTP hydrolysis, leaving RAS predominantly in the GTP-bound state, the active state [3]. For many years, drugs targeting KRAS directly did not exist because of its small size, smooth surface, and strong binding affinity for GTP (with KRAS able to bind with GTP at picomolar concentrations) [4,5].

Research of Related Drugs
Currently, there are three strategies for targeting KRAS mutation. One is to develop drugs that can compete with GTP. However, we found two problems with this strategy in our study: the first problem is the high affinity between GTP and KRAS, the second is the high concentration of GTP in cells in vivo (as well as the low specific binding of GTP analogs to KRAS) [6]. Another strategy is to prevent KRAS from combining with GEFs, which leads to the activation of KRAS. For example, Burns MC et al [7] found a small molecule that can combine with SOS1, which provided a basis for Xu L et al [8] to find related inhibitors. The third strategy is to change the location of the KRAS protein. The key to this strategy is PDEδ, which inhibits the activation of the ERK and KRAS signaling pathways. Currently, some groups are preparing to develop related small-molecule drugs [9].

EARLY STUDIES
In 2013, Shoket and his colleagues [10] proposed the idea of targeting the 12th cysteine of mutant KRAS. They set up a screening model of disulfide fragments based on the structure of KRAS G12C in the GTP-bound state and identified 2E07 (18) and 6H05 (19), both of which bind to the switch II pocket. This finding laid the foundation for further research. In 2014, Lim et al [11] proposed the molecule SML-10-70-1 in a paper published in Angewandte Chemie; SML-10-70-1 was able to achieve a small decrease in pERK and pAkt at 100 μM and had no effect on wildtype KRAS. However, it also affected KRAS G12 S mutant cells, suggesting that it has off-target effects. Lito's group [12] further investigated a KRAS G12C covalent binding inhibitor called ARS-853 (See Figure 4). They found that ARS-853 could only interact  with KRAS G12C in the GDP-bound state by comparing the therapeutic effect of ARS-853 on cells with KRAS G12C in the GTP-bound state or GDP-bound state. The nucleotide state of KRAS G12C is in dynamic flux, and ARS-853 fully integrates with the protein and inhibit its activation by modulating the upstream signaling factor [13,14]. The disadvantages of ARS-853 are its poor stability in plasma (t 1/2 < 20 min) and its low bioavailability after oral administration in mice (F < 2%). As such, ARS-853 has not been further studied.

ARS-1620
The Mechanism of Action of ARS-1620 After improving the core structure of ARS-853, Janes et al [15] synthesized a quinazoline core as a versatile lead scaffold. Among the drugs using this scaffold, the representative drug is ARS-1620 (See Figure 5). ARS-1620 covalently modified KRAS G12C at a rate of 1,100 ± 200 M −1 s −1 (kobs/[I]), 10 times higher than the rate of ARS-853. In H358 cell lines, ARS-1620 inhibited RAS-GTP binding and the phosphorylation of MEK, ERK, RSK, S6, and AKT in a dose-dependent and selective manner but had no effect on the negative control group (A549, H460, and H44 cells), indicating that it selectively inhibited G12C mutant cells but not wild-type cells. Moreover, ARS-1620 showed excellent oral bioavailability (F >60%) and had sufficient plasma stability in mice. To further determine whether ARS-1620 has sufficient covalent and pharmacokinetic properties to enable it to function in vivo, the researchers administered ARS-1620 to a subcutaneous xenograft model with KRAS G12C-bearing tumors at a dose of 200 mg/kg/day and found that the tumor obviously receded.

Combination Therapies Including ARS-1620
Using ARS-1620 alone confers drug resistance to tumor cells and leads to tumor recurrence after regression. Miriam et al [16] found that deletion of MTOR genes can make cells significantly sensitive to KRAS and IGF1R inhibitors. The researchers also found that blocking the IGF1R, MAPK, and PI3K/AKT/mTOR signaling pathways simultaneously led to the death of cancer cells carrying KRAS mutations. On the basis of previous findings, they screened the entire genome (16,019 genes) in shRNA-infected KRAS-mutant non-small-cell lung cancer (NSCLC) H23 cells, trying to identify triple combination therapies using ARS-1620, mTOR inhibitors and the IGF1R inhibitor linsitinib. They found that triple therapy could significantly reduce tumor size in mice and humans, and cell growth was not detected for 17 days. Signaling pathway analysis showed not only that ARS-1620 inhibited ERK and S6 phosphorylation but also that the triple therapy persistently inhibited AKT and S6 phosphorylation. In addition, the triple therapy reduced the reactivation of RAS and ERK activation for 48 h.

AMG-510
The Mechanism of Action of AMG-510 Due to the small size of the switch II pocket, additional proteinligand interactions are limited; this limitation makes further improvements in ARS-1620 difficult. However, with the discovery of the obvious hydrogen bonding between ARS-1620 and His95, new progress has been achieved by researchers. A surface groove formed by the alternative orientation of His95 can be occupied by aromatic rings, and this discovery might enhance the interaction between drugs and KRAS G12C [17]. Therefore, a new drug called AMG-510 (See Figure 6), which has similar ligand structure to ARS-1620, was developed by Cannon et al. [18] However, His95 is a newly identified site for AMG-510 binding. AMG-510 almost completely inhibited pERK (IC50 ≈ 0.03 μM) in NCI-H358 and MIA PaCa-2 cells after 2 h of treatment, and its IC50 value was 20 times that of ARS-1620. In terms of cell viability, AMG-510 decreased the viability of NCI-H358 and MIA PaCa-2 cells (IC50 ≈ 0.006 μm and 0.009 μm, respectively), with IC50 values approximately 40 times those of ARS-1620. However, the strong binding affinity of AMG-510 may covalently link it to other cysteine-containing proteins,  which is a problem that needs to be addressed in the clinical trial stage [19].

Clinical Application of AMG-510
In a patient trial (four patients with NSCLC), the researchers found that treatment with AMG-510 resulted in objective partial responses (PRs) in two patients and stable disease (SD) in two patients.

AMG-510 Combination Therapy
The clinical results of strategies combining BRAF and MEK inhibitors [23] suggest that combinations with AMG-510 and other inhibitors may show enhanced tumor cell killing effects and overcome drug resistance. One team conducted experiments in vitro in KRAS G12C cell lines using AMG-510 and HER kinase, EGFR, SHP2, PI3K, AKT, and MEK inhibitors and found that the combination of AMG-510 and MEK inhibitors significantly enhanced antitumor activity. In an AMG-510 monotherapy mouse experiment, the tumors of mice lacking T cells were likely to recur. The researchers used a model called CT-26KRASG12C [24,25], which is widely used to assess the effectiveness of immunotherapy and combinations of immunotherapy and targeted drugs, to assess AMG-510 combined with a PD-1 inhibitor. As a result, 1/10 of the tumors completely disappeared when AMG-510 or the PD-1 inhibitor was used alone. However, 9/10 of the tumors disappeared completely with the combination therapy. Treatment was stopped after the 43rd day, and no recurrence was seen in any complete responder after 112 days.

MRTX849
The Mechanism of Action of MRTX849 Hallin et al [26] discovered MRTX849 (See Figure 7)

Clinical Application of MRTX849
The preliminary results of a phase I/II trial, which began in January 2019, were first presented at the AACR-NCI-EORTC Conference in 2019 (International Conference on Molecular Targets and Cancer Therapy) [27]. Of the 12 assessable patients (6 with NSCLC, 4 with colorectal cancer (CRC) and 2 with appendix cancer), 3 (50%) NSCLC patients and 1 (25%) CRC patient achieved a PR. The most common adverse events from MRTX849 were grade 1/2 diarrhea or nausea, and no other serious adverse reactions have yet been found.

MRTX849 Combination Therapy
MRTX849 combined with HER family inhibitors synergistically inhibited tumor cell viability in most cell lines and was first tested in combined screening in vitro. The researchers selected afatinib as a representative HER family inhibitor and chose five tumor models (KYSE410, SW1573, H2122, H2030, and LU6405 cells) with partial sensitivity to single-agent MRTX849 or refractoriness to the treatment, and all the cell lines showed obvious antitumor activity. They found that HER family activation in vivo may limit MRTX849-mediated inhibition of the ERK and mTOR-S6 signaling pathways, while administration of afatinib combined with MRTX849 could limit feedback reactivation of ERK signaling and showed complementary inhibition of AKT-mTOR-S6 signaling, significantly enhancing antitumor activity. Moreover, the combination of SHP2 inhibitors and MRTX849 also achieved inhibition of tumor activity. Administration of the mTOR inhibitor vistusertib with MRTX849 induced complementary inhibition of the ERK and mTOR-S6 signaling pathways, showing extensive antitumor activity.

BI-2852
Kessler et al [28] developed BI-2852 (See Figure 8), which is different from the drugs mentioned above; it binds to the RAS switch I/II pocket with nanomolar-level affinity (recent rabbit experiments [29,30] have found that it modulates GEFs, GAPs and downstream effector interactions). In addition, BI-2852 can interact with KRAS in both the GDP-and GTP-bound states, which in turn blocks interactions of all GEFs, GAPs and effectors with KRAS and prevents the propagation of downstream signaling pathways and KRAS proliferation.

ARS-3248
Wellspring Biosciences and Janssen recently obtained approval for research of their new KRAS G12C inhibitor ARS-3248, which is based on ARS-1620. ARS-3248 will be tested in a phase 1 trial, and no research data have been published yet.

Other New Drugs
LY3499446, tetrahydroquinazoline derivatives (US 2019/ 0248767A1) and tetracycline compounds (WO 2019/110751A1) are also currently in the R&D phase of development. Among the three, LY3499446 appears to be further in the development process, and a phase I study related to LY3499446 has begun to recruit patients in Australia. LY3499446 will be assessed as monotherapy in this study and used in combination with other drugs, including abemaciclib, cetuximab, and erlotinib, in advanced solid tumors (including NSCLC and CRC) [31].

CHALLENGES
KRAS inhibitors are in phase I clinical trials, and some early data have showed inactivity of some cancer cells after treatment, while others avoid this effect to restore proliferation. Lito et al [32] found that some KRAS receptor tyrosine kinases can trigger nucleotide exchange. AURK signal transduction promotes effector activation and cell cycle progression; cells with new KRAS G12C mutation can quickly escape inhibition and resume proliferation, and these cells can rapidly change to an active state that is insensitive to drugs. KRAS inhibitors are a new promising tool, and an increasing number of related drugs will be developed. However, most of these agents lack data to prove their clinical effectiveness, and their side effects are still unclear. We cannot yet determine whether KRAS G12C inhibitors can achieve maximum effects as monotherapy, and the current combinations including KRAS G12C inhibitors do not have guaranteed lasting efficacy. In addition, new challenges will likely be encountered in the future.

AUTHOR CONTRIBUTIONS
AY collected data and wrote the manuscript, ML and MF helped to revise the manuscript.